Technical Data
A2294-30G
Angiostatin
Description:
Embryonic vascular system undergoes a series of complex, highly regulated series of events involving differentiation, migration and association of primitive endothelial cells. This process is termed vasculogenesis. A further remodeling of the primitive vascular system forms the mature cardiovascular system. This process is known as angiogenesis (sprouting of new capillary vessels from pre-existing vasculature). Angiogenesis accounts for the formation of vasculature into previously avascular organs such as brain and kidney. Angiogenic activity in the adult is required during the normal tissue repair, and for the remodeling of the female reproductive organs (ovulation and placental development). Certain pathological conditions, such as tumor growth and diabetic retinopathy, also require angiogenesis.

Recent studies have identified several proteolytic fragments or cryptic domains of proteins that act as inhibitors of angiogenesis. These include fragments of plasminogen such as Angiostatin protein (kringles 1-4) and kringles 1-5, C-terminal proteolytic fragment of Collagen XVIII (Endostatin protein), the NC10 domain of collagen 15 (Restin), the C-terminal hemopexin-like domain of MMP-2 (PEX), the N-terminal fragment of prolactin, and the N-terminally truncated platelet factor. Angiostatin protein, a proteolytic fragment of plasminogen, is comprised of the first four kringle regions. It prevents the growth of endothelial cells, and its systemic administration inhibits the growth of primary carcinomas in mice. Kringles 1-3 fragment has a greater inhibitory activity than the kringles 1-4 fragment. The protease-activated kringles 1-5 is the most potent plasminogen fragment with over 50-fold greater endothelial cell specific inhibitory activity. Its systemic administration inhibited the growth of fibrosarcoma and significantly reduced neovascularization.

Applications:
Suitable for use in Western Blot and ELISA. Other applications not tested.

Recommended Dilution:
Western Blot: 1-5ug/ml (chemiluminescence)
ELISA: 1:10,000-1:100,000; 50-100ng control peptide/well
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG11C79Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
Purified angiostatin protein (kringles 1-4) protein (~50kD).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity:
Recognizes human Angiostatin. Species Crossreactivity: does not crossreact with mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Peterson Te et al (1990) JBC 265, 6104-6111. 2. Forsgren m et al (1987) FEBS Lett. 213, 254-260. 3. Malinowski:DP et al (1984) Biochemistry 23, 4243-4250. 4. OReilly MS et al 1994) Cell 79, 315-328. 5. Sim BK et al (1997) Cancer Res. 57, 1329-1334. 6. Wu Z et al (1997) BBRC 236, 651.