Technical Data
A2294-48K1
Angiotensin I (Angiotensin-1, Ang I)
Description:
Angiotensin is an oligopeptide in the blood that causes vasoconstriction, increased blood pressure, and release of aldosterone from the adrenal cortex. It is a hormone and a powerful dipsogen. It is derived from the precursor molecule angiotensinogen, a serum globulin produced in the liver. It plays an important role in the renin-angiotensin system.

Angiotensin I is formed by the action of renin on angiotensinogen. Renin is produced in the kidneys in response to both decreased intra-renal blood pressure at the juxtaglomerular cells, or decreased delivery of Na+ and Cl- to the macula densa. If more Na+ is sensed, renin release is decreased. Renin cleaves the peptide bond between the leucine (Leu) and valine (Val) residues on angiotensinogen, creating the 10aa peptide (des-Asp) angiotensin I. Angiotensin I appears to have no biological activity and exists solely as a precursor to angiotensin II.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000-1:10,000 (50-100ng of control peptide/well)
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a11C80Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
Synthetic peptide corresponding to aa40-49 of the angiotensinogen precursor (Swiss-Prot entry# P01019) (HMW).
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity:
Recognizes human Angiotensin I. Does not react with angiotensin II, III or angiotensinogen in ELISA.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Martin MM et al 1995 BBRC 209, 554-562. 2. Chassagne C et al 1995 Genomics 25, 601-603. 3. Koike G ett al 1994 BBRc 203, 1842-50. 4. Tsuzuki S et al 1994 BBRC 200,1449-54. 5. Martin MM et al 1994 BBRC 205, 645-651. 6. Lazard D et al 1994 Receptor Channels 2, 271-280.