Technical Data
A2294-48P
AGT, CT (Angiotensinogen, Serpin A8, Angiotensin-1, Angiotensin I, Ang I, Angiotensin-2, Angiotensin II, Ang II, Angiotensin-3, Angiotensin III, Ang III, Des-Asp[1]-angiotensin II, SERPINA8)
Description:
AGT, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia. Mutations in AGT gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in AGT gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.

Applications:
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide selected from the C-terminal region of human AGT (KLH).
Purity:
Purified by ammonium sulfate precipitation.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human AGT.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Gurkan,A., Arch. Oral Biol. 54(4), 337-344 (2009). 2.Vickers,C., J. Biol. Chem. 277(17), 14838-14843 (2002). 3.Donoghue,M., Circ. Res. 87(5), E1-E9 (2000).