Technical Data
A2295-01D
Angiotensin Converting Enzyme (ACE, CD143)
Description:
Applications:
Suitable for use in Immunohistochemistry, Immunocytochemistry, Western Blot, Immunoprecipitation and ELISA. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry: 1:1000 on fresh frozen or 4% paraformaldehyde fixed tissues. Does not work on paraffin embedded tissue.
Immunocytochemistry: 1:100. Reacts with endothelial cell, macrophages (activated) and THP1 cells (monocyte line).
Western Blot: 1:100
Immunoprecipitation: 1:1000-1:10,000
ELISA: 1:1000
Optimal dilutions to be determined by the researcher.

Matched Pair:
Suitable for use with A2295-01C in a sandwich ELISA.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG12Q2314Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Angiotensin-converting enzyme from human lung.
Purity:
Purified immunoglobulin
Form
Supplied as a liquid in PBS, pH 7.4, 150mM sodium chloride containing 0.05% sodium azide.
Specificity:
Recognizes N-terminal epitope of Angiotensin-converting enzyme (ACE). Species Crossreactivity: Human, monkey, rat, hamster and feline.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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