Technical Data
A2295-01L
Angiotensin Converting Enzyme (ACE, ACE1, DCP1)
Description:
Renin-Angiotensin System (RAS) is a crtical regulator of blood pressure homeostasis. The protease renin cleaves angiotensinogen into inactive decameric peptide angiotensin-I (Ang-I). Angiotensin-converting enzyme (ACE) then cleaves C-terminal dipeptide from Ang-I to form an active octamer angiotensin-II (Ang-II), which can contribute to hypertension by promoting vascular smooth muscle vasocontriction and renal tubule sodium reabsorption. ACE can also cleave many other small peptides including the vasodialating peptide bradykinin into inactive fragment, cleave Alzheimer amyloid beta-peptide (Abeta), retard Abeta aggregation, deposition and fibril formation. ACE mutant mice display spontaneous hypotension, partial male infertility and kidney malformations. ACE is found in somatic (s-ACE) and testicular/germinal (t-ACE) isoforms. The products of renin and ACE catalysis, namely Ang1-10 and Ang1-8 can also be by another peptidase, ACE-2 to Ang1-9 and Ang1-7, respectively. ACE-2 and ACE (s-ACE and t-ACE) are made as transmembrane (TM) proteins.

These enzymes also exist as soluble, truncated forms lacking the TM and cytosolic domains. ACE (also known as dipeptidyl carboxypeptidase-1, DCP1; Kininase-II, ACE1) gene has been mapped at human chromosome 17q23. The s-ACE and t-ACE isoforms are generated by alternative splicing of ACE-2 gene. Somatic-ACE, a Zn (II) containing dipeptidyl carboxy peptidase is a single chain glycoprotein with a molecular mass of ~140kD. The s-ACE enzymes from mouse (1312aa), rat (1313aa) and human (1306aa) contain two large areas of homologous sequence, each containing catalytic site and a Zn-binding region. These homologous regions are approximately half the size of whole s-ACE. The s-ACE is expressed in many somatic tissue tissues, including vascular endothelial cells, renal epithelial cells, and testicular Leydig cells.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-10ug/ml (ECL). Recognizes >120kD somatic-ACE from native tissue.
ELISA: 0.5-1ug/ml. Coat plates at 1ug/ml with A2295-01K
Angiotensin Converting Enzyme, Mouse, Control Peptide. Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4░C for short-term only. For long-term storage, store at -20░C. Aliquots are stable for at least 12 months at -20░C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4░C (-20░C Glycerol)Blue IceMouseRabbit
Concentration:
~1mg/ml
Immunogen:
A synthetic peptide corresponding to15aa located in the extracellular region of mouse somatic-ACE (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.5, 0.1% BSA, 40% glycerol.
Specificity:
Recognizes mouse Angiotensin Converting Enzyme. Species sequence homology: human-87% and rat-.81% No significant sequence homolgy exist with the t-ACE of any species.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Crackower, M.A., Nature 417: 822 (2002). 2.áSibinga, N.E.S., et al., Circ. Res. 87: e1-e9 (2000). 3.áBernstein, K.E., et al., JBC 264: 11,945 (1989). 4.áTinis, S.R., et al., JBC 275: 33,238 (2000).