Technical Data
A2295-01W9
Angiotensin Converting Enzyme 2 (ACE-2, ACEH)
Description:
Renin-Angiotensin System (RAS) is a critical regulator of blood pressure homeostasis. The protease renin cleaves angiotensinogen into inactive decameric peptide angiotensin-I (Ang-I). Angiotensin-converting enzyme (ACE) then cleaves a C-terminal dipeptide from Ang-I to form an active octamer angiotensin-II (Ang-II), which can contribute to hypertension by promoting vascular smooth muscle vasoconstriction and renal tubule sodium reabsorption. ACE mutant mice display spontaneous hypotension, partial male infertility and kidney malformations. ACE is found in somatic (s-ACE) and testicular/germinal (t-ACE) isoforms.
The ACE-2 gene has been mapped at human chromosome Xp22. ACE-2 enzymes from human (805aa) and mouse (798aa) are single chain proteins with 40% seq homology to N- and C-terminal domains of ACE. However, in contrast to s-ACE, which consists of two catalytic sites, ACE-2 contains only one active site. Unlike s-ACE and t-ACE, which are dipeptidyl-carboxypeptidases, ACE-2 acts as a carboxypeptidase, cleaving a single residue from Ang-I, generating Ang1-9 and a single residue from Ang-II to generate Ang1-7. ACE-2 can cleave angiotensin-I but not bradykinin and the enzyme activity is not inhibited by the ACE inhibitors. This enzyme is expressed highly in heart, kidney and testis, and moderately in colon, small intestine and ovary. ACE-2 is an essential regulator of heart function because targeted disruption of this enzyme in mice results in severe cardiac contractility defect, increased angiotensin-II levels and upregulation of hypoxia-induced genes in the heart.

Applications:
Suitable for use in Western Blot and ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:10K-1:30K
Western Blot: 1-2ug/ml
Optimal dilutions to be determined by researcher.

Positive Control:
A2295-01J1, Angiotensin Converting Enzyme 2, Recombinant, Human

Hybridoma:
Purified NSO-derived recombinant human angiotensin I converting enzyme-2 (rhACE-2) ectodomain (aa18-740).

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a7C9Affinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Purified NSO-derived recombinant human angiotensin I converting enzyme-2 (rhACE-2) ectodomain (aa18-740).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes human ACE-2. Does not crossreact with ACE-1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Tipnis, S. R et al (2000) JBC Vol. 275: 33238-33243; 2. Crackower, M. A et al (2002), Nature 417: 822-828; 3. Huang, L et al (2003) JBC Vol. 278: 15532-15540.