Technical Data
Angiotensin Converting Enzyme 2 (ACE-2, ACEH)
ACE-2 (also known as ACE-2 and ACE homolog, ACEH) gene has been mapped at human chromosome Xp22. ACE-2 enzymes from human (805aa) and mouse (798aa) are single chain proteins with 40% sequence homology to N- and C-terminal domains of ACE. However, in contrast to s-ACE which consists of two catalytic sites, ACE-2 contains only one active site. Unlike s-ACE and t-ACE which are dipeptidyl-carboxypeptidases, ACE-2 acts as a carboxypeptidase, cleaving single residue from Ang-I, generating Ang1-9 and a single residue from Ang-II to generate Ang1-7. ACE-2 can cleave angiotensin-I, but not bradykinin and the enzyme activity is not inhibited by the ACE inhibitors. This enzyme is expressed highly in heart, kidney and testis and moderately in colon, small intestine and ovary. ACE-2 is an essential regulator of heart fuction because targeted disruption of this enzyme in mice results in severe cardiac contractility defect, increased angiotensin-II levels and upregulation of hypoxia-induced genes in the heart.

Suitable for use in Flow Cytometry, ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA (Direct and Indirect): 1:2000-1:10,000
Western Blot: 1:2000-1:10,000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween-20 and 5% skim milk; suggested incubation time is 1 hour at RT. Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
50ug-20CBlue IceHumanMouse
Recombinant human ACE2 (angiotensin-converting enzyme 2).
Supplied as a liquid in PBS, pH 7.4. No preservatives.
Recognizes human ACE2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. A. Zulli, et al., Exp. Physiol. 93: 564 (2008).