Description:
Recommended Secondary Reagents: F(ab')2 rabbit anti-mouse IgG HRP, F(ab')2 rabbit anti mouse IgG: RPE conjugate
Fusion Partners: Spleen cells from immunized Balb/c mice were fused with cells of the mouse X36-Ag8-653 myeloma cell line.
Recommended Negative Controls: Mouse IgG1 Negative Control
Applications:
Flow Cytometry: Neat -1:10
Immunohistochemistry: (frozen)- Neat-1:30
ELISA
Immunoprecipitation
Optimal working dilutions to be determined by researcher.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.
Immunohistochemistry: Recommended positive control tissue: Kidney.
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| Type | Isotype | Clone | Grade |
|---|
| Mab | IgG1 | 3H534 | Supernatant |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 1ml | | -20°C | Blue Ice | Human | Mouse |
|
| Concentration: |
| As reported |
| Immunogen: |
| Human lung CD143 (Angiotensin converting enzyme). |
| Purity: |
| Tissue culture supernatant. |
| Form |
| Tissue Culture Supernatant. Supplied as a liquid with 0.1% sodium azide. |
| Specificity: |
| Recognizes human CD143, also known as angiotensin-converting enzyme (ACE). CD143 exists in two forms, a 170kD somatic form and a 90kD germinal form. The somatic form is expressed by endothelial cells (especially those of arterioles and lung capillaries), epithehal cells (especially in proximal renal tubules and in the small intestine), by some neuronal cells and variably on some macrophages and T lymphocytes. The germinal form is expressed by spermatozoa. This antibody recognizes active ACE binding to an N-terminal domain epitope different to that recognized by clone 9B9. Species Crossreactivity: Human, monkey |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
1. Danilov, S.M. et al. (1994). Structure - funstion analysis of angiotensin - converting enzyme using monoclonal antibodies. Selective inhibition of N-domain active center. J. Biol. Chem. 269: 26806 - 26814. 2. Metzger, R.
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