Recommended Secondary Reagents: F(ab')2 rabbit anti-mouse IgG HRP, F(ab')2 rabbit anti mouse IgG: RPE conjugate
Fusion Partners: Spleen cells from immunized Balb/c mice were fused with cells of the mouse X63-Ag8-653 myeloma cell line.
Recommended Negative Controls: Mouse IgG1 Negative Control
Flow Cytometry: Neat -1:10
Optimal working dilutions to be determined by researcher.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.
Immunohistochemistry: Recommended Positive Control Tissue: Kidney.
| As reported|
|Human lung CD143 (Angiotensin converting enzyme).|
|Tissue culture supernatant.|
|Tissue Culture Supernatant. Supplied as a liquid with 0.1% sodium azide.|
|Recognizes human somatic CD143, also know as angiotensin-converting enzyme (ACE). CD143 exists in two forms, a 170kD somatic form and a 90kD germinal form. The somatic form is expressed by endothelial cells (especially those of lung capillaries and arterioles), epithelial cells (especially in proximal renal tubules and small intestine), by some neuronal cells and variably by some macrophages and T lymphocytes. The germinal form is expresssed by spermatozoa. This antibody recognizes active ACE binding to an N-terminal domain epitope, different to that recognized by clone i2H5. Species Crossreactivity: Human, monkey (3), rat (3), hamster, feline |
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Danilov, S. M. et al. (1994). Structure - Function analysis of angiotensin - converting enzyme using monoclonal antibodies. Selective inhibition of N-domain active center. J. Biol. Chem. 269: 26806 – 26814 2. Metzger, R. e|