Technical Data
A2295-02B
Angiotensin Converting Enzyme (ACE, CD143)
Description:
Recommended Secondary Reagents: F(ab')2 rabbit anti-mouse IgG HRP, F(ab')2 rabbit anti mouse IgG: RPE conjugate

Fusion Partners: Spleen cells from immunized Balb/c mice were fused with cells of the mouse X63-Ag8-653 myeloma cell line.

Recommended Negative Controls: Mouse IgG1 Negative Control

Applications:
Flow Cytometry: Neat -1:10
Immunohistochemistry (Frozen)
ELISA
Immunoprecipitation
Optimal working dilutions to be determined by researcher.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.
Immunohistochemistry: Recommended Positive Control Tissue: Kidney.
TypeIsotypeCloneGrade
MabIgG13H535Supernatant
SizeStorageShippingSourceHost
1ml-20CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
Human lung CD143 (Angiotensin converting enzyme).
Purity:
Tissue culture supernatant.
Form
Tissue Culture Supernatant. Supplied as a liquid with 0.1% sodium azide.
Specificity:
Recognizes human somatic CD143, also know as angiotensin-converting enzyme (ACE). CD143 exists in two forms, a 170kD somatic form and a 90kD germinal form. The somatic form is expressed by endothelial cells (especially those of lung capillaries and arterioles), epithelial cells (especially in proximal renal tubules and small intestine), by some neuronal cells and variably by some macrophages and T lymphocytes. The germinal form is expresssed by spermatozoa. This antibody recognizes active ACE binding to an N-terminal domain epitope, different to that recognized by clone i2H5. Species Crossreactivity: Human, monkey (3), rat (3), hamster, feline
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Danilov, S. M. et al. (1994). Structure - Function analysis of angiotensin - converting enzyme using monoclonal antibodies. Selective inhibition of N-domain active center. J. Biol. Chem. 269: 26806 26814 2. Metzger, R. e