Technical Data
A2295-02C
Angiotensin Converting Enzyme (ACE, CD143)
Description:
CD143 exists in two forms, a 170kD somatic form and a 90kD germinal form. The somatic form is expressed by endothelial cells (especially those of lung capillaries and arterioles), epithelial cells (especially in proximal renal tubules and small intestine), by some neuronal cells and variably by some macrophages and T lymphocytes. The germinal form is expresssed by spermatozoa. Study of CD143 may be relevant to fertility (especially sperm-egg interactions), atherosclerosis and in drug targeting to the lung.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:50-1:100
Immunohistochemistry (Paraffin): Neat. 1:20. Requites antigen retrieval using heat treatment methods prior to staining.
Optimal dilutions to be determined by the researcher.

Hybridoma:
Spleen cells from immunized Balb/c mice were fused with cells of the mouse X63-Ag8-653 myeloma cell line.

Recommended Positive Control Tissue:
Kidney (lung in rat studies)

Recommended Negative Control:
I1904-79G: IgG1, Mouse, Negative Control

Recommended Secondary Reagents:
I1904-65W: IgG, F(ab)2 (HRP) Pab RbxMo
I1904-66C: IgG, F(ab)2, X-Adsorbed (HRP) Pab RbxMo

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol 40-50%, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG13H536Supernatant
SizeStorageShippingSourceHost
1ml4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
Not Determined
Immunogen:
Denatured Human kidney CD143 (angiotensin converting enzyme).
Purity:
Tissue culture supernatant
Form
Supplied as a liquid with 0.1% sodium azide.
Specificity:
Recognizes human CD143, also know as angiotensin-converting enzyme (ACE). This clone recognizes denatured ACE, binding to an epitope in the C domain and has been reported to be active in Western Blot. Species Crossreactivity: monkey, rabbit , bovine, canine, feline, guinea pig, rat, porcine and mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Metzger, R. et al. (1999) Angiotensin-converting enzyme in non-neoplastic kidney diseases Kidney International 56: 1442-1454. 2. Balyasnikova, I.V. et al. (2003) Monoclonal antibodies to denatured ACE (CD143) broad species specificity, reactivity on paraffin sections and detection of subtle conformational changes in the C-terminal domain of ACE. Tissue Antigens. 61 (1): 49- 62.