		Technical Data
A2295-02E	Angiotensin Converting Enzyme (ACE, CD143) (Biotin)

Description:
CD143 exists in two forms, a 170kD somatic form and a 90kD germinal form. The somatic form is expressed by endothelial cells (especially those of arterioles and lung capillaries), epithehal cells (especially in proximal renal tubules and in the small intestine), by some neuronal cells and variably on some macrophages and T lymphocytes. The germinal form is expressed by spermatozoa.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: Neat.1:10. Use 10ul to label 10e6 cells in 100ul.
Optimal dilutions to be determined by the researcher.

Hybridoma:
Spleen cells from immunized Balb/c mice were fused with cells of the mouse X36-Ag8-653 myeloma cell line.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	3H534	Purified

Size		Storage	Shipping	Source	Host
100ug		4°C (-20°C Glycerol)	Blue Ice	Human	Mouse

Concentration:

~0.1mg/ml

Immunogen:

Human lung CD143 (Angiotensin converting enzyme).

Purity:

Purified

Form

Supplied as a liquid, 0.1% sodium azide, 1% BSA. Labeled with Biotin.

Specificity:

Recognizes human CD143, also known as angiotensin-converting enzyme (ACE). Also recognizes active ACE binding to an N-terminal domain epitope different to that recognized by clone 9B9. Species Crossreactivity: monkey

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Danilov, S.M. et al. (1994). Structure - funstion analysis of angiotensin - converting enzyme using monoclonal antibodies. Selective inhibition of N-domain active center. J. Biol. Chem. 269: 26806 - 26814. 2. Metzger, R.