		Technical Data
A2295-02F	Angiotensin Converting Enzyme (ACE, CD143) (Biotin)

Description:
CD143 exists in two forms, a 170kD somatic form and a 90kD germinal form. The somatic form is expressed by endothelial cells (especially those of lung capillaries and arterioles), epithelial cells (especially in proximal renal tubules and small intestine), by some neuronal cells and variably by some macrophages and T lymphocytes. The germinal form is expresssed by spermatozoa.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:10 (neat). Use 10e6 cells in 100ul.
Optimal dilutions to be determined by the researcher.

Hybridoma:
P3X63-Ag8.653. Myeloma cells with spleen cells from Balb/c mice.

Recommended Negative Control:
I1904-78M: Mouse IgG1 Negative Control

Recommended Secondary Reagent:
S7973-96B: Streptavidin: FITC

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	3H539	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		4°C (-20°C Glycerol)	Blue Ice	Human	Mouse

Concentration:

~0.1mg/ml.

Immunogen:

Human lung CD143.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in 0.09% sodium azide, 1% BSA. Labeled with Biotin.

Specificity:

Recognizes human CD143 (ACE). Reacts with active ACE by binding to an N-terminal domain epitope, different to that recognized by clone i2H5. Species Crossreactivity: Monkey, rat, hamster, feline.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Danilov, S. M. et al . (1994). Structure - Function analysis of angiotensin - converting enzyme using monoclonal antibodies. Selective inhibition of N-domain active center. J. Biol. Chem. 269: 26806 - 26814 2. Metzger, R. et al . (2000). CD143 in the development of atherosclerosis. Atherosclerosis. 150: 21 - 31 3. Danilov, S. et al . (1991). Lung is the target organ for a monoclonal antibody to angiotensin-converting enzyme. Lab. Invest. 64 (1): 118 - 124.