Technical Data
A2295-02S
Angiotensin Converting Enzyme 2 (ACE-2, ACEH)
Description:
Angiotensin-converting enzyme 2 (ACE2) plays a central role in vascular, renal, and myocardial physiology (1-2). In contrast to its homolog ACE, ACE2 expression is restricted to heart, kidney, and testis. Recently. ACE2 has also been shown to be a functional receptor of the SARS coronavirus (3). The normal function of ACE2 is to convert the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1-7, unlike ACE, which converts AngI to AngII. While the role of these vasoactive peptides is not well understood, lack of ACE2 expression in ace2-/ace2- mice leads to severely reduced cardiac contractility, indicating its importance in regulating heart function (4).

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-2.0ug/ml.
Optimal dilutions to be determined by the researcher.

Positive Control: Human kidney or human testis cell lysate.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide corresponding to amino acids near the center of human ACE2.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes human ACE2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Donoghue M, et al (2000) Circ. Res. 87:1-9. 2. Tipnis SR, et al (2000) J Biol. Chem. 275:33238-43. 3. Li W, et al (2003) Nature. 426:450-4. 4. Crackower MA, et al (2002)