Technical Data
A2295-08D
Angiotensin II Type 1 Receptor (AT1)
Description:
Angiotensin II mediates its action by interacting with membrane receptor, Angiotensin II type 1 receptors. The signal is transmitted via G-proteins that activates a phosphatidylinositol- calcium second messenger system. ATII receptors are integral membrane proteins of approx.359-363aa and predicted to contain at least 7 transmembrane domains. The N and C-terminus are predicted to extracellular and cytoplasmic, respectively. Three isoforms of ATII receptors have been cloned. In rat type 1a (359 aa) is expressed in the liver kidney, aorta, lung, uterus, ovary, spleen, heart, adrenal and vascular smooth muscles (1). Rat Type 1b (also known as AT3; 359 aa) is found in liver, kidney, and aorta (2). Type 1c (177 aa) has been detected in brain, kidney, liver, spleen and lung (3).

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-10ug/ml using ECL. Detects ~45-kD band.
ELISA: 1:10,000-1:100,000 using 50-100ng of A2295-08K control peptide/well.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1906 IgY, Chicken Pab Rb x Ch
I1906-10 IgY, Chicken (HRP) Rb x Ch
I1906-12 IgY, Chicken (FITC) Rb x Ch
I1906-14 IgY, Chicken (AP) Rb x Ch

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgYAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRatChicken
Concentration:
~1mg/ml
Immunogen:
A 10aa peptide sequence mapping near the N-terminus of rat AT II (KLH).
Purity:
Purified by affiinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity:
Recognizes rat Angiotensin II Type 1 Receptor. Species sequence homology: mouse, rat, human, ovine, porcine, canine, chimp, chicken.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Stewart JA; 2006; J Mol Cell Cardiol, 41, 97-107; WB; 2. Hoffman A; 2004; J. Recept Signal Transduction, 23 1; WB; IHC 3. Javeshghani D; 2003; Hypertension, 42, 761-767; WB; 4. Nadal JA; 2002; Am. J. Physiol 282, H739-H748; WB,; 5. Javeshghani D; 2003; Hypertension, 42, 761-767; WB; 6. Zeng C; 2002; Hypertension, 41, 724-729; WB; 7. Jin X-Q; 2002; Hypertension. 39:1021-1027; WB; 8. Nithipatikom K; 2004; AJPEM, 287: 820 - 827; WB,; IF 9. Su J-Z; 2002; Hypertension : 40, 853-858; WB; 10. Javeshghani D; 2003; Hypertension, 42, 761-767; WB; 11. Hussain T; 1998; Hypertension 32: 1054-1059; WB,; 12. Rodriguez-Iturbe B; 2001; Kidney Intl. 59, 6, 2222; ; IHC, 13. Lee Jongun; 2002; Pharmacological Res. 46, 5, 383-387; WB; 14. Hakam AC; 2006; AJEP, 290: F503 - F508.; WB; 15. Murphy Tj et al (1991) Nature 351, 233; 16. Iwai N et al (1991) BBRC 177, 299; 17. Langford K et al (1992) BBRC 183, 1025; 18. Sasamura h et al (1992) BBRC 185, 253; 19. Yoshida H et al (1992) BBRC 186, 1042; 20. Mauzy CA et al (1992) BBRC 183, 8; 21. Bergsma DJ et al (1992) BBRC 183, 989 22. Konishi H et al (1992) BBRC 199, 467; 23. Iwai N et al (1992) FEBS Lett. 298, 257; 24. Sandberg K et al (1992) BBRC 183, 1090; 25. Elton TS et al (1992) BBRC 184, 106726. Hahn AW et al (1993) BBRC 192, 1260.