Technical Data
A2295-13A
Anion Exchanger 1 (AE1)
Description:
Anion exchangers (AE) are membranes proteins involved in the regulation of intracellular pH, cell volume regulation as well as in transepithelial acid/base transport. AE proteins are sodium-independent exchangers that mediates one-for-one exchange of extracellular Cl- for intracellular HCO3- ions resulting in intracellular acidification. AE proteins are encoded by a family of at least three related genes (AE1, AE2 and AE3). Numerous alternatively spliced isoforms of each AE gene are expressed in various tissues. AE proteins are exemplified by a large N-terminal cytoplasmic domain (~40-75kD) that provides binding sites for cytoskeleton protein, glycolytic enzymes and hemoglobin. The N-terminal cytoplasmic domains of AE2 are AE3 are more closely related than AE1. In fact, AE1 N-terminus is 300aa shorter than both the AE2 and AE3. The C-terminal TM domain (~55kD) is highly conserved (~70% identity) among various AE, spans the lipid bilayer 12-14 times. It is able to mediate anion exchange by itself. AE1 protein (also know as band 3) is the most abundant protein in red cell membrane. AE1 gene has been cloned and characterized from a variety of species including mouse, rat, human and chicken.

Applications:
Suitable for use in Western Blot and ELISA. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1-10ug/ml (ECL.) AE1 has a molecular weight of ~90-100kD. It aggregates easily forming dimers and multimers. It may also be proteolytically processed to smaller fragments of 60, 40 and 20kD.
ELISA: 1-10ug/ml. Using 1ug/ml A2295-13B4 control peptide per well.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4C (-20C Glycerol)Blue IceRatRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to a 20aa sequence near the N-terminus of rat AE1 (KLH coupled).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.5, 0.2% BSA.
Specificity:
Recognizes rat Anion Exchanger 1 (AE1). No significant sequence homology with other AE or other proteins. Species Sequence Homology: Mouse: 85%; human: 70%; bovine: 75%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kudrycki KE et al (1989) J Biol Chem. 264:8185. 2. Kopito RR et al (1985) Nature 316:234. 3. Raida M et al (1989) BBA 980:291. 4. Lux SE et al (1989) PNAS 86:9089. 5. Tanner MJ et al (1988) Biochem. J. 256:703.