		Technical Data
A2295-13B1	Anion Exchanger 2 (AE-2)

Description:
Human AE2 gene (chromosome 7q35-q36) encodes a protein of 1241 aa (mouse 1237 aa, rat 1234 aa). Several isoforms of AE2 are also expressed as a result of alternative splicing. AE2 is widely expressed in many tissues.

Applications:
Suitable for use in ELISA, Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-10ug/ml using ECL.
ELISA: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.

Control Peptide:
A2295-14D1 Anion Exchanger 2, Rat Control Peptide (AE2)

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
50ug		4°C (-20°C Glycerol)	Blue Ice	Rat	Rabbit

Concentration:

~1mg/ml

Immunogen:

A 21 AA Peptide sequence near the N-terminus of rat AE-2 (KLH).

Purity:

Purified by affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 40% glycerol.

Specificity:

Recognizes rat Anion Exchanger 2. Species Crossreactivity: mouse. Species sequence homology: rabbit (90%), human (85%), chicken (80%).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Negrini C et al (1995) BBA 1236, 207; Chow A et al (1992) Am J Physiol. 263, G345; Kudrycki KE et al (1990) J Biol. Chem. 265, 462; Kopito et al (1990) Intl. Rev. Cytol. 123, 177; Lindsey AE et al (1990) PNAS 87, 3456; Alper et al (1988) J Biol. Chem. 263, 17092; Demuth DR et al (1986) EMBO J., 5, 1205.