Technical Data
A2295-13B2
Anion Exchanger 1 (AE1)
Description:
Anion exchangers (AE) are membranes proteins involved in the regulation of intracellular pH, cell volume regulation as well as in transepithelial acid/base transport. AE proteins are sodium-independent exchangers that mediates one-for-one exchange of extracellular Cl- for intracellular HCO3- ions resulting in intracellular acidification. AE proteins are encoded by a family of at least three related genes (AE1, AE2, and AE3). Numerous alternatively spliced isoforms of each AE gene are expressed in various tissues. AE proteins are exemplified by a large N-terminal cytoplasmic domain (~40-75kD) that provides binding sites for cytoskeleton protein, glycolytic enzymes and hemoglobin. The N-terminal cytoplasmic domains of AE2 are AE3 are more closely related than AE1. In fact, AE1 N-terminus is 300 aa shorter than both the AE2 and AE3. The C-terminal TM domain (~55kD) is highly conserved (~70% identity) among various AE, spans the lipid bilayer 12-14 times, and is able to mediate anion exchange by itself.
AE1 protein (also know as band 3) is the most abundant protein in red cell membrane. AE1 gene has been cloned and characterized from a variety of species including mouse, rat, human, and chicken. Human AE1 gene (chromosome 17q21-qter) encodes for a protein of 911 aa (rat 927 aa; mouse 929 aa). Several isoforms of AE1 are also transcribed. AE1 is primarily expressed in red cells and kidney. AE1 is dimeric in solution. Defects in AE1/band3 gene are the cause of hereditary ovalocystosis (HO)-a disease associated with high degree of membrane rigidity.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:5000. AE1 is approx 90-100kD proteins. It aggregates easily forming dimers and multimers. It may also be proteolytically processed to smaller fragments of 60, 40, 20kD.
ELISA: 1:10,000-50,000. Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.

Control Peptide:
A2295-13B4, Anion Exchanger 1, Rat, Control Peptide (AE1)

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul4C (-20C Glycerol)Blue IceRatRabbit
Concentration:
Not determined
Immunogen:
An 20 AA Peptide sequence near the N-terminus of rat AE-1 (1). The peptide was coupled to KLH
Purity:
Serum
Form
Supplied as a liquid, neat serum, 0.05% sodium azide, 40% glycerol.
Specificity:
Recognizes rat Anion Exchanger 1 (AE1). Species sequence homology: mouse 90%; human 75%; bovine 70%. No significant sequence homology is detected with other AE or other proteins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kudrycki KE et al (1989) J Biol Chem. 264, 8185; Kopito RR et al (1985) Nature 316, 234; Raida M et al (1989) BBA 980, 291; Lux SE et al (1989) PNAS 86, 9089; Tanner MJ et al (1988) Biochem. J. 256, 703.