Technical Data
Anion Exchanger 1 (AE1)
Anion exchangers (AE) are membranes proteins involved in the regulation of intracellular pH, cell volume regulation as well as in transepithelial acid/base transport. AE proteins are sodium-independent exchangers that mediates one-for-one exchange of extracellular Cl- for intracellular HCO3- ions resulting in intracellular acidification. AE proteins are encoded by a family of at least three related genes (AE1, AE2, and AE3). Numerous alternatively spliced isoforms of each AE gene are expressed in various tissues. AE proteins are exemplified by a large N-terminal cytoplasmic domain (~40-75kD) that provides binding sites for cytoskeleton protein, glycolytic enzymes and hemoglobin. The N-terminal cytoplasmic domains of AE2 are AE3 are more closely related than AE1. In fact, AE1 N-terminus is 300 aa shorter than both the AE2 and AE3. The C-terminal TM domain (~55kD) is highly conserved (~70% identity) among various AE, spans the lipid bilayer 12-14 times, and is able to mediate anion exchange by itself.

AE1 protein (also know as band 3) is the most abundant protein in red cell membrane. AE1 gene has been cloned and characterized from a variety of species including mouse, rat, human, and chicken. Human AE1 gene (chromosome 17q21-qter) encodes for a protein of 911 aa (rat 927 aa; mouse 929 aa). Several isoforms of AE1 are also transcribed. AE1 is primarily expressed in red cells and kidney. AE1 is dimeric in solution. Defects in AE1/band3 gene are the cause of hereditary ovalocystosis (HO)-a disease associated with high degree of membrane rigidity.

Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1-10ug/ml using ECL.
ELISA: 0.5-1ug/ml Control peptide can be used to coat ELISA plates at 1ug/ml.
Immunohistochemistry: 2-10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug4C (-20C Glycerol)Blue IceRatRabbit
An 20 AA Peptide sequence near the N-terminus of rat AE-1 (1). The peptide was coupled to KLH
Purified over the peptide-Sepharose column
Supplied as a lyophilized powder from PBS, pH 7.4, 0.1% BSA.
The 20 AA rat control peptide is 90% conserved in mouse, 75% in human and 70% in bovine AE1. No significant sequence homology is detected with other AE or other proteins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kudrycki KE et al (1989) J Biol Chem. 264, 8185; Kopito RR et al (1985) Nature 316, 234; Raida M et al (1989) BBA 980, 291; Lux SE et al (1989) PNAS 86, 9089; Tanner MJ et al (1988) Biochem. J. 256, 703.