Technical Data
A2295-14D
Anion Exchanger 2 (AE2)
Description:
Anion exchangers (AE) are membranes proteins involved in the regulation of intracellular pH, cell volume regulation as well as in transepithelial acid/base transport. AE proteins are sodium-independent exchangers that mediates one-for-one exchange of extracellular Cl- for intracellular HCO3- ions resulting in intracellular acidification. AE proteins are encoded by a family of at least three related genes (AE1, AE2, and AE3). Numerous alternatively spliced isoforms of each AE gene are expressed in various tissues. AE proteins are exemplified by a large N-terminal cytoplasmic domain (~40-75kD) that provides binding sites for cytoskeleton protein, glycolytic enzymes and hemoglobin. The N-terminal cytoplasmic domains of AE2 are AE3 are more closely related than AE1. In fact, AE1 N-terminus is 300 aa shorter than both the AE2 and AE3. The C-terminal TM domain (~55kD) is highly conserved (~70% identity) among various AE, spans the lipid bilayer 12-14 times, and is able to mediate anion exchange by itself.
Human AE2 gene (chromosome 7q35-q36) encodes a protein of 124 aa (mouse 1237 aa, rat 1234aa). Several isoforms of AE2 are also expressed as a result of alternative splicing. AE2 is widely expressed in many tissues.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:5000
ELISA: 1:10,000-50,000
Optimal dilutions to be determined by the researcher.

Control Peptide:
A2295-14D1 Anion Exchanger 2, Rat Control Peptide

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul4C (-20C Glycerol)Blue IceRatRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to a 21 AA Peptide sequence near the N-terminus of rat AE-2 (KLH).
Purity:
Serum
Form
Supplied as a liquid in PBS and 0.05% sodium azide, 40% glycerol.
Specificity:
Recognizes rat AE-2. Species sequence homology: mouse- 100%, rabbit-90%, human-85%, chicken-80%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Negrini C et al (1995) BBA 1236, 207; 2. Chow A et al (1992) Am J Physiol. 263, G345; 3. Kudrycki KE et al (1990) J Biol. Chem. 265, 462; 4. Kopito et al (1990) Intl. Rev. Cytol. 123, 177; 5. Lindsey AE et al (1990) PNAS 87, 3456; 6. Alper et al (1988) J Biol. Chem. 263, 17092; 7. Demuth DR et al (1986) EMBO J., 5, 1205.