		Technical Data
A2295-14F	Anion Exchanger 3 (AE3, Anion exchange protein 3, CAE3/BAE3, Cardiac/brain band 3-like protein, Neuronal band 3-like protein, SLC2C, Solute carrier family 4 member 3)

Description:
Anion exchangers (AE) are membranes proteins involved in the regulation of intracellular pH, cell volume regulation as well as in transepithelial acid/base transport. AE proteins are sodium-independent exchangers that mediates one-for-one exchange of extracellular Cl- for intracellular HCO3- ions resulting in intracellular acidification. AE proteins are encoded by a family of at least three related genes (AE1, AE2, and AE3). Numerous alternatively spliced isoforms of each AE gene are expressed in various tissues. AE proteins are exemplified by a large N-terminal cytoplasmic domain (~40-75kD) that provides binding sites for cytoskeleton protein, glycolytic enzymes and hemoglobin. The N-terminal cytoplasmic domains of AE2 are AE3 are more closely related than AE1. In fact, AE1 N-terminus is 300 aa shorter than both the AE2 and AE3. The C-terminal TM domain (~55kD) is highly conserved (~70% identity) among various AE, spans the lipid bilayer 12-14 times, and is able to mediate anion exchange by itself. Human AE3 gene (chromosome 2q36) encodes a protein of 1233 aa (mouse/rat 1227 aa). AE3 is primarily expressed in excitable tissue such cardiac muscle, retina, and brain.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:5000 (ECL)
ELISA: 1:10,000-50,000. A2295-14F2 Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Serum

Size		Storage	Shipping	Source	Host
50ul		-20°C	Blue Ice	Rat	Rabbit

Concentration:

Not Determined

Immunogen:

Synthetic 20aa peptide sequence near the N-terminus, Cytoplasmic domain of rat AE-3 (KLH). Species sequence homology: mouse: 100%; human: 95%; rabbit: 90%.

Purity:

Serum

Form

Supplied as a liquid in PBS, 0.05% sodium azide.

Specificity:

Recognizes rat AE3. No significant sequence homology is detected with other AE or other proteins. Does not recognize cardiac AE3.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Kudrycki KE et al (1990) J Biol. Chem. 265, 462. 2. Kopito RR et al (1989) Cell 59, 927. 3. Morgan CW et al (1993) J Cell Sci. 106, 1275. 4. Yannoukakos D et al (1994) Cir. Res. 75, 603. 5. Kopito RR et al (1990) Intl. Rev. Cytol. 123, 177.