Technical Data
A2295-14G
Anion Exchanger 4 (AE4)
Description:
Anion exchangers (AE) are membrane proteins involved in the regulation of intracellular pH, cell volume regulation as well as in transepithelial acid/base transport. AE proteins are sodium-independent exchangers that mediates one-for-one exchange of extracellular Cl- for intracellular HCO3- ions resulting in intracellular acidification. AE proteins are encoded by a family of at least three related genes (AE1, AE2, and AE3). Numerous alternatively spliced isoforms of each AE gene are expressed in various tissues. AE proteins are exemplified by a large N-terminal cytoplasmic domain (~40-75kD) that provides binding sites for cytoskeleton protein, glycolytic enzymes and hemoglobin. The N-terminal cytoplasmic domains of AE2 are AE3 are more closely related than AE1. In fact, AE1 N-terminus is 300aa shorter than both the AE2 and AE3. The C-terminal TM domain (~55kD) is highly conserved (~70% identity) among various AE, spans the lipid bilayer 12-14 times, and is able to mediate anion exchange by itself.
Recently, a new member of AE family, AE4, has been cloned from rabbit and human. Human AE4 (945aa; chromosome 5) is predicted to have 14 TM domains. It is strongly expressed in the kidney. Rabbit and human AE4 share 84% identity.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:10,000-50,000. Coat ELISA plates at 1ug/ml with A2295-14G2-Anion Exchanger 4, Control Peptide Human.
Western Blot: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to 15aa near the cytoplasmic C-terminus of human AE4 (KLH).
Purity:
Serum
Form
Supplied as a liquid in PBS, 0.05% sodium azide, before the addition of glycerol to 40%.
Specificity:
Recognizes human AE4. Sequence Homology: Human isoforms 1AE, 2, 3, NBC5 (AB032762, 657aa) and NBC (AF313465, 990aa; XM_038736, 959aa) - 100%. Species Sequence Homology: Rabbit and rat AE4 - 100%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Parker, M.D., et al., BBRC 282: 1103-1109 (2001). 2. Tsuganezawa, H., et al., J. Biol. Chem. 276: 8180-8189 (2001).