Technical Data
Anion Exchanger 4 (AE4)
Anion exchangers (AE) are membranes proteins involved in the regulation of intracellular pH, cell volume regulation as well as in transepithelial acid/base transport. AE proteins are sodium-independent exchangers that mediates one-for-one exchange of extracellular Cl- for intracellular HCO3- ions resulting in intracellular acidification. AE proteins are encoded by a family of at least three related genes (AE1, AE2, and AE3). Numerous alternatively spliced isoforms of each AE gene are expressed in various tissues. AE proteins are exemplified by a large N-terminal cytoplasmic domain (~40-75kD) that provides binding sites for cytoskeleton protein, glycolytic enzymes and hemoglobin. The N-terminal cytoplasmic domains of AE2 are AE3 are more closely related than AE1. In fact, AE1 N-terminus is 300aa shorter than both the AE2 and AE3. The C-terminal TM domain (~55kD) is highly conserved (~70% identity) among various AE, spans the lipid bilayer 12-14 times, and is able to mediate anion exchange by itself.

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-10ug/ml (ECL)
ELISA: 0.5-1ug/ml. Coat ELISA plates at 1ug/ml with A2295-14G2 Anion Exchanger 4, Control Peptide Human.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
50ug-20CBlue IceHumanRabbit
As reported
Synthetic peptiide corresponding to 15aa near the cytoplasmic C-terminus of human AE4 (KLH).
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, 0.1% BSA.
Recognizes human AE4. Sequence homology: human isoforms AE1-AE3, NBC5, NBC. Species sequence homology (100%): rat and rabbit.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Parker MD et al (2001) BBRC 282, 1103-1109; Tsuganezawa H et al (2001) JBC 276, 8180-8189