Technical Data
A2295-15G1
ANXA7, ID (Synexin, Annexin VII, Annexin-7, Annexin A7, ANX7, SNX, OK/SW-cl.95)
Description:
Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins.The Annexin VII gene contains 14 exons and spans approximately 34 kb of DNA. An alternatively spliced cassette exon results in two mRNA transcripts of 2.0 and 2.4 kb which are predicted to generate two protein isoforms differing in their N-terminal domain. ANXA7 is a protein with a molecular weight of approximately 51kD with a unique, highly hydrophobic N-terminal domain of 167 amino acids and a conserved C-terminal region of 299 amino acids. The latter domain is composed of alternating hydrophobic and hydrophilic segments. Structural analysis of the protein suggests that Annexin VII is a membrane binding protein with diverse properties, including voltage-sensitive calcium channel activity, ion selectivity and membrane fusion.

Applications:
Suitable for use in ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Flow Cytometry: 1:10-1:50
Immunofluorescence: 1:10-1:50
Immunohistochemistry: 1:50-1:100
Western Blot: 1:100-1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
KLH-conjugated synthetic peptide corresponding to a portion of amino acids within aa335-365 in the center region of human ANXA7
Purity:
Purified by Protein G affinity chromatography
Form
Supplied as a liquid
Specificity:
Recognizes human ANXA7
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Shirvan,A., et.al., Biochemistry 33(22), 6888-6901 (1994).