Technical Data
ANXA1 (Annexin A1, ANXA1, ANX1, LPC1, Lipocortin I) (Control Peptide)
Molecular Biology Storage: -20CShipping: Blue Ice
C-terminus of aa-CQAILDETKGDYEK.
Control peptide corresponding to polyclonal antibody, Catalog#A2295-15N, goat x .

Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
Suitable for use in Peptide ELISA. Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can

Peptide Blocking (see corresponding antibody A2295-15N):
Antibodies are typically supplied at 0.5mg/ml and peptides as a 100ul pellet. When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
Source: Synthetic
Purity: Highly Purified
Concentration: ~0.5mg/ml
Form: Supplied as a lyophilized powder in PBS. Reconstitution: Reconstitute with 200ul sterile ddH2O.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Fan X, Krahling S, Smith D, Williamson P, Schlegel RA. Macrophage surface expression of annexins I and II in the phagocytosis of apoptotic lymphocytes. Mol Biol Cell. 2004 Jun;15(6):2863-72. Epub 2004 Apr 2.
PMID: 15064349

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.