Technical Data
Annexin I
The Annexins are a structurally related family of calcium-binding proteins that associate reversibly with membranes, and bind to phospholipid bilayers in the presence of micromolar free calcium concentrations. This binding is speci c for calcium and for acidic phospholipids. Each of the annexin proteins consist of an N-terminal domain of variable length followed by four or eight copies of a conserved segment of sixty one residues. The repeat (sometimes known as an ‘endonexin fold’) consists of ve alpha-helices that are wound into a right-handed superhelix. Annexins have been demonstrated to be involved in cytoskeletal interactions, phospholipase inhibition, intracellular signaling, anticoagulation, and membrane fusion. Although the annexins are primarily intracellular proteins and lack hydrophobic signal sequences, some family members (e.g. Annexin I and V) have been found to be secreted.
Annexin I (calpactin II, chromobindin 9, GIF, Lipocortin, p35) is a cytoskeletal calcium-dependent phospholipid-binding protein which has been shown to be a mediator of corticosteroid activity, a substrate for serine/threonine kinases and growth regulated tyrosine kinases, and it may be involved in secretion. Although this protein has received considerable attention due to its potential involvement in a variety of pathways, research progress on the localization of Annexin I during development and in pathophysiological conditions has been hampered due to con icting reports on the immunolocalization of the protein. These contradictory data are believed to result in large part from unusual properties of the protein and its apparent sensitivity to different xation conditions.

Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Positive Control Lysates:
Human broblasts, mouse kidney, mouse lung, adult rat and mouse brain

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20°CBlue IceHumanRabbit
Not Determined
Full-length recombinant protein corresponding to human Annexin I purifued from yeast.
Supplied as a liquid, 0.09% sodium azide.
Recognizes human Annexin I protein and does not crossreact with other Annexins. Reactivity with Annexin I was confirmed by Western Blot using total lysates derived from human fibroblasts. Species Crossreactivity: mouse and rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Wallner, B.P., et al., Nature 320: 77-81 (1986). 2. Varticovski, L., et al., Biochemistry 27: 3682-3690 (1988). 3. Biemann, K., et al., Science 237: 992-998 (1987). 4. Arcone, R., et al., Eur. J. Biochem. 211: 347-355 (1993). 5. Raynal, P., et al., Biochim. Biophys. Acta 1197: 63-93(1994). 6. Barton, G.J., et al., Eur. J. Biochem. 198: 749-760 (1991). 7. Klee, C.B., Biochemistry 27: 6645-6653 (1988). 8. Smith, P.D., et al., Trends Genet. 10: 241-246 (1994). 9. Huber, R., et al., EMBO J. 9: 3867-3874 (1990). 10. Mckanna, J.A., et al., J. Cell. Phys. 153: 467-476 (1992). 11. Strijbos, P.J., et al., Brain Res. 553: 249-260 (1991). 12. Mullens, L., et al., J. Neuroimmunol. 50: 25-33 (1994). 13. Mckanna, J.A., J. Neurosci. Res. 36: 491-500 (1993).