Technical Data
ANKTM1 (TRPA1, Transient Receptor Potential Cation Channel, subfamily A, Member 1, Ankyrin-like with transmembrane domains protein 1, Transformation-sensitive protein p120, Transient receptor potential cation channel subfamily A member 1)
Transient receptor potential ion channels (TRPCs) are a superfamily of six transmembrane segment-spanning, gated cation channels. TRPA1 is a TRP-related channel that responds to cold temperatures and pungent compounds and plays a role in both nociceptor and hair cell transduction. It is a transformation-associated gene product in lung epithelia, whereas its protein distribution is primarily restricted to sensory neurons. Blocking TRPA1 may be a therapeutic target for treating cold hyperalgesia caused by inflammation and nerve damage. It is now known that TRPA1 protein is also widely expressed outside of the CNS and is dys-regulated during oncogenic transformation.

Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence/Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 2ug/ml. Detects a band at ~110kD.
Immunocytochemistry (IF): 1:200
Immunohistochemistry (Paraffin): 1:100-1:250
Immunohistochemistry: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ul-20CBlue IceHumanRabbit
Synthetic peptide corresponding to a region within the N-terminus (residues 1-100) of the human TRPA1 protein [Swiss-Prot#O75762].
Purified by affinity chromatography
Supplied as a liquid in Tris-glycine, 150mM sodium chloride, 0.05% sodium azide.
Recognizes human ANKTM1. Species Crossreactivity: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.