Technical Data
A2295-19J1
ANK1 (Ankyrin-1, Ankyrin 1, ANK-1, Ankyrin-R, Erythrocyte Ankyrin, ANK)
Description:
Ankyrins are a family of proteins that link the integral membrane proteins to the underlying spectrin actin cytoskeleton and therefore play a role in cell motility, proliferation and intercellular contact. Different isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue specific, developmentally regulated manner. Most ankyrins are typically composed of three structural domains. Ankyrin 1, the prototype of this family, was first discovered in erythrocytes but has also been found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated with hereditary spherocytosis. Different isoforms of ankyrin 1 have been described. Alternative polyadenylation accounting for the different sized erythrocytic ankyrin 1 mRNAs, has also been reported.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:128,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-QIVKRASLKRGKQ, from ANK1, at C-terminal (NP_000028.3, NP_065208.2, NP_065209.2, NP_065210.2, NP_065211.2, NP_065213.2).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity:
Recognizes ANK1. Species sequence homology: Bovine, human, mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Dubreuil RR. Functional links between membrane transport and the spectrin cytoskeleton. J Membr Biol. 2006;211(3):151-61. Epub 2006 Nov 7. Review.