		Technical Data
A2295-19W	Ankyrin repeat and SOCs box-containing protein, mouse Asb-5 (Asb-11)

Description:
The ankyrin repeat and SOCS box-containing (Asb) protein family is comprised of members which each contain “SOCS boxes” but differ from other families of protein in the motifs they contain upstream of the SOCS box(1). SOCS boxes are carboxy-terminal regions of homology found in the suppressor of cytokine signaling family of proteins. The box region is thought to be the point of interaction between SOCS proteins and E3 ubiquitin ligases.

Applications:
Suitable for use in Western Blot and ELISA. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.

Positive Control:
Human liver whole cell lysate

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
200ul		4°C (-20°C Glycerol)	Blue Ice	Human	Rabbit

Concentration:

Not determined

Immunogen:

Synthetic peptides corresponding to aa40-51 and aa250-265 of human Asb-11.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, 0.1mg/ml BSA, 0.02% sodium azide.

Specificity:

Recognizes human Ankyrin repeat and SOCs box-containing protein, mouse Asb-5 (Asb-11).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Kile, BT., et al.: Cloning and characterization of the genes encoding the ankyrin repeat and SOCS box-containing proteins Asb-1, Asb-2, Asb-3 and Asb-4. Gene 258(1-2): 31-41 (2000). 2. Krebs, D.L., Hilton, D.J., Stem Cells 19: 378-87 (2001). 3. Wang, J., Campbell, I.L.. J. Neurosci. Res. 67: 423-7 (2002).