Technical Data
A2295-23
Annexin II
Description:
Annexin II (calpactin I, chromobindin 8, p36, Lipocortin II, PAP-IV, or Protein I) is a cytoskeletal calcium-dependent phospholipid binding protein, which has been shown to be a mediator of cortocosteriod activity, a substrate for serine/threonine kinases and growth regulated tyrosine kinases, and may play a role in secretion.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 5-10ug/ml
Western Blot: 0.5-1.0ug/ml
ELISA: 0.1-1.0ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
A431 Cell Lysate

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG13G9Affinity Purified
SizeStorageShippingSourceHost
100ug4°C (-20°C Glycerol)Blue IceBovineMouse
Concentration:
~0.25mg/ml
Immunogen:
Annexin II puri ed from bovine lung.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.5, 0.1% BSA, 1.5mM sodium azide, 50% glycerol.
Specificity:
Recognizes bovine Annexin II. Does not crossreact with other Annexin proteins. Species Crossreactivity: human, sheep and canine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Creutz, C.E., et al., J. Biol. Chem. 262:1,860 (1987). 2. Zokas, L. and Glenney, Jr., J.R., J. Cell Biol., 105: 2,111 (1987). 3. Feinberg, J.M., et al., J. Histochem. and Cytochem., 39: 955 (1991).