Technical Data
A2296-82A
Anterior Gradient 2 (AG-2, AGR2, Secreted Cement Gland Protein XAG-2 Homolog, HPC8)
Description:
AG-2 and AG-3 are recently discovered human homologues of the secreted Xenopus laevis proteins XAG-1/2 (AGR-1/2) that are expressed in the cement gland, an ectodermal organ in the head associated with anteroposterior fate determination during early development(1). Although the roles of AG-2 and AG-3 in mammalian cells are unknown, both proteins share a high degree of protein sequence homology and lie adjacent to one another on chromosome 7p21.

Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:1000
Immunohistochemistry (Paraffin): 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.


TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa55-72, TQTYEEALYKSKTSNKPL, of human Anterior Gradient 2 (Genbank accession no. NP_006399.1) Cellular Localization: Secreted
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.05% sodium azide, 0.2% gelatin.
Specificity:
Recognizes human Anterior Gradient 2. Species Sequence Homology: mouse (88%) and bovine (94%)
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Fletcher GC et al. hAG-2 and hAG-3, human homologues of genes involved in differentiation, are associated with oestrogen receptor-positive breast tumours and interact with metastasis gene C4.4a and dystroglycan. Br J Cancer 88:579-85 (2003). Petek E et al. Localization of the human anterior gradient-2 gene (AGR2) to chromosome band 7p21.3 by radiation hybrid mapping and fluorescencein situ hybridisation. Cytogenet Cell Genet 89:141-2 (2000).