		Technical Data
A2296-82A	Anterior Gradient 2 (AG-2, AGR2, Secreted Cement Gland Protein XAG-2 Homolog, HPC8)

Description:
AG-2 and AG-3 are recently discovered human homologues of the secreted Xenopus laevis proteins XAG-1/2 (AGR-1/2) that are expressed in the cement gland, an ectodermal organ in the head associated with anteroposterior fate determination during early development(1). Although the roles of AG-2 and AG-3 in mammalian cells are unknown, both proteins share a high degree of protein sequence homology and lie adjacent to one another on chromosome 7p21.

Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:1000
Immunohistochemistry (Paraffin): 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide corresponding to aa55-72, TQTYEEALYKSKTSNKPL, of human Anterior Gradient 2 (Genbank accession no. NP_006399.1) Cellular Localization: Secreted

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, 0.05% sodium azide, 0.2% gelatin.

Specificity:

Recognizes human Anterior Gradient 2. Species Sequence Homology: mouse (88%) and bovine (94%)

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

Fletcher GC et al. hAG-2 and hAG-3, human homologues of genes involved in differentiation, are associated with oestrogen receptor-positive breast tumours and interact with metastasis gene C4.4a and dystroglycan. Br J Cancer 88:579-85 (2003). Petek E et al. Localization of the human anterior gradient-2 gene (AGR2) to chromosome band 7p21.3 by radiation hybrid mapping and fluorescencein situ hybridisation. Cytogenet Cell Genet 89:141-2 (2000).