Technical Data
A2296-82A2
Anterior Gradient 2 (AG-2, AGR2, Secreted Cement Gland Protein XAG-2 Homolog, HPC8)
Description:
The anterior gradient protein-2 (AGR2) is a cancer cell marker specifically up-regulated in response to depletion of serum and oxygen. It has been identified as a tumor marker in primary and secondary cancer lesions, and as a marker for detection of circulating tumor cells. Elevated levels of AGR2 are known to increase the metastatic potential of cancer cells. Specifically, AGR2 may serve as a useful molecular marker and/or potential therapeutic target for hormone-responsive breast tumors. AGR2 expression promotes tumor growth in esophageal adenocarcinoma cells and intestinal cells of secretory lineage, suggesting that AGR2 may be important for the growth and development of the intestine as well as esophageal adenocarcinomas.

Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1:20
Western Blot: 1:5000-1:10,000
Immunoprecipitation: 1:20
Immunohistochemistry: 1:250-1:500
Immunocytochemistry: 1:100-1:250
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG9H291Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
A synthetic peptide corresponding to residues in human AGR2
Purity:
Supernatant
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.01% sodium azide, 50% glycerol
Specificity:
Recognizes human AGR2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Zweitzig DR, et al. Mol Cell Biochem. 306(1-2):255-60, 2007 2. Fletcher GC, et al. Br J Cancer 88(4):579-85, 2003 3. Wang Z, et al. Cancer Res. 68(2):492-7, 2008