Technical Data
Anterior Gradient 2, ID (Anterior Gradient Protein 2 Homolog, AG-2, hAG-2, HPC8, Secreted Cement Gland Protein XAG-2 Homolog, AGR2, AG2, UNQ515/PRO1030)
AG-2 (Anterior gradient protein 2; also AGR2, GOB4 and HPC8) is an 18-21kD member of the PDI family of enzymes. It is expressed in secretory cell types, such as prostate epithelium, small intestine goblet, Paneth and enteroendocrine cells, and multiple carcinoma cell types. Although AG-2 possesses a signal sequence, it appears to be rarely secreted, instead being found in the ER due the presence of a KTEL ER retention signal at its C-terminus. AG-2 forms transient disulfide linkages with molecules destined for secretion, possibly aiding protein folding. Mature human AG-2 is 155aa in length (aa21-175). Cys81 is presumed to participate in intermolecular bond formation. There is one potential alternative start site 20aa upstream of the standard start site.

Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:128,000
Western Blot: 1:128,000
Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
50ug-20CBlue IceHumanGoat
As reported
Synthetic peptide corresponding to C-GAKKDTKDSRPK from an internal region of human AGR2.
Purified by immunoaffinity chromatography.
Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Recognizes human AGR2. Species sequence homology: Canine and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.