Technical Data
A2296-84A
APH1, NT (Gamma-secretase Subunit APH-1A, APH-1a, APH-1a, Aph-1alpha, Presenilin-stabilization Factor, PSF, CGI-78, UNQ579/PRO1141)
Description:
APH1 is an essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein). The gamma-secretase complex is minimally composed of a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PEN2, although other components may exist. APH1 probably represents a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex.

Applications:
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide selected from the N-terminal region of human APH1 (KLH).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human APH1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Ota, T., et al., Nat. Genet. 36(1):40-45 (2004). 2.Clark, H.F., et al., Genome Res. 13(10):2265-2270 (2003). 3.Marlow, L., et al., Biochem. Biophys. Res. Commun. 305(3):502-509 (2003). 4.Kimberly, W.T., et al., Proc. Natl. Acad. Sci. USA 100(11):6382-6387 (2003). 5.Edbauer, D., et al., Nat. Cell Biol. 5(5):486-488 (2003).