Technical Data
Media
Antibiotic Medium #3 (Powder)
A2297-27
Microbiological Grade
Storage RT/4C    Shipping RT
Components shown as g/liter
Beef Extract1.5
Dextrose1.0
Pancreatic Digest of Gelatin5.0
Potassium Phosphate, Dibasic3.68
Potassium Phosphate, Mono1.32
Sodium Chloride3.5
Yeast Extract1.5
Total per Liter:17.5g
Dehydrated culture media suitable for the microbial assay and detection of antibiotics in milk and plate counts in pharmaceutical products. Formulated in acordance with the specifications and protocols as listed by the FDA, Grove and Randall and USP 23.

The activity of an antibiotic can be demonstrated under suitable conditions by its inhibitory effect on organisms. Antibiotic assays are performed by the cylinder plate method and the turbidimetric assay. These media has been formulated according to the specifications and procedures listed by the Food and Drug Administration, European Pharmacopeia, Code of Federal Regulations, and the USP.

Directions per Liter:
Dissolve 17.5 grams per liter of distilled/deionized water, heating with stirring until completely solubilized. Dispense into appropriate containers, loosen caps and autoclave for 15-30 minutes at 121C (15 psi).

Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.


Directions per Liter: Dissolve 17.5 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi).

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

Appearance: Light beige, homogeneous and free flowing powder

Solubility: Yellow to medium amber and turbid to clear.

pH: As Reported


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. CRF:21, 300.50 2. Bacteriological Analytical Manual, Sixth Ed. (1984) 3. Assay Methods of Antibiotics, Grove, D.C. and Randall, W.A., Medical Encyclopedia Inc., N.Y. (1955) 4. USP XXI, Rockville, MD (1985)