Technical Data
Media
Antibiotic Medium #11 (Powder)
A2297-36
Microbiological Grade
Storage RT/4C    Shipping RT
Components shown as g/liter
Pancreatic Digest of Casein17.0
Pancreatic Digest of Gelatin6.0
Beef Extract1.5
Yeast Extract3.0
Dextrose1.0
Agar15.0
Total:43.5g/liter
The activity of an antibiotic can be demonstrated under suitable conditions by its inhibitory effect on organisms. Antibiotic assays are performed by the cylinder plate method and the turbidimetric assay. These media has been formulated according to the specifications and procedures listed by the Food and Drug Administration, European Pharmacopeia, Code of Federal Regulations, and the USP. The Antibiotic Media are identified numerically and also, where applicable, with names assigned by Grove and Randall in Assay Methods of Antibiotics. Antibiotic Medium #11 is recommended for determing antibotic potency to neymycin.

Storage and Stability:
Store the sealed bottle containing the dehydrated medium at RT. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light beige color.


Directions: 1. Dissolve 43.5grams per liter of distilled/deionized water, heating to boiling with stirring until completely solubilized.

2. Dispense into appropriate containers, loosen caps and autoclave for 15-30 minutes at 121C (15 psi). Storage:Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

Appearance: Light beige, homogeneous, free flowing to containing soft lumps.

Solubility: Light to medium amber, clear, complete with heat

pH (3.1% solution at 25C): 7.9 0.2

Microbiological Analysis: Micrococcus luteus (ATCC 9341)


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. CRF:21, 300.50 2. Bacteriological Analytical Manual, Sixth Ed. (1984) 3. Assay Methods of Antibiotics, Grove, D.C. and Randall, W.A., Medical Encyclopedia Inc., N.Y. (1955) 4. USP XXI, Rockville, MD (1985)