Technical Data
A2298-13A
Antiplasmin, alpha2
Description:
alpha 2Antiplasmin (alpha,2AP), also known as alpha 2Plasmin Inhibitor (alpha 2PI), is a member of the SERPIN family of proteinase inhibitors and the primary inhibitor of the enzyme plasmin in blood. It is produced in the liver and circulates in plasma at ~70ug/ml (~1uM). alpha 2AP is a single chain molecule with a mass of 67kD as determined by SDS-PAGE. The primary target enzyme for alpha 2AP is plasmin, but alpha 2AP also acts as secondary or “backup” inhibitor of activated F.XI, activated Protein C and trypsin. Inhibition of these enzymes by alpha 2AP occurs by proteolytic cleavage after Arg364 with subsequent rapid formation of a stable, inactive 1:1 enzyme-alpha 2AP complex. alpha 2AP also acts to regulate fibrinolysis by binding to the lysine binding sites on plasminogen thus competitively inhibiting plasminogen binding to fibrin. About 30% of alpha 2AP present in plasma is partially degraded and lacks a peptide in the carboxyl region that contains the plasminogen-binding site. This form of alpha 2AP (~65kD) has a reduced rate of plasmin inhibition and has been referred to as the “slow form” of alpha 2AP. During fibrin formation, a portion of circulating alpha 2AP is cross-linked to the alpha -chain of fibrin by activated factor XIII, and this linking of plasmin inhibitor to the plasmin substrate provides an additional measure of protection to the fibrin clot from proteolysis by plasmin.

Applications:
Suitable for use in Immunoelectrophoresis and ELISA. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
500ug4°C (-20°C Glycerol)Blue IceHumanGoat
Concentration:
~2mg/ml
Immunogen:
Human Antiplasmin, alpha2 (alpha2AP) purified from plasma.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 10mM HEPES, pH 7.4, 0.15M sodium chloride, 50% glycerol.
Specificity:
Recognizes human Antiplasmin, alpha2. Assay by Immunoelectrophoresis has one arc with normal plasma. No arc was seen with alpha2AP deficient plasma.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Aoki N, Suni Y, Miura O, Hirosawa S; Human alpha 2Plasmin Inhibitor; Methods in Enzymology, 223, pp 185-197, 1993. 2. Shieh BH, Travis J; The Reactive Site of Human alpha 2-Plasmin Inhibitor; JBC 262, pp 6055-6059, 1987. 3. Moroi M, Aoki N; Isolation and Characterization of alpha 2-Plasmin Inhibitor from Human Plasma; JBC 251, pp 5956-5965, 1976. 4. Harpel PC; Blood Proteolytic Enzyme Inhibitors: Their Role in Modulating Blood Coagulation and Fibrinolytic Enzyme Pathways; in Hemostasis and Thrombosis, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 738-747, J.B. Lippincott Co., Philadelphia PA, USA, 1982.