Technical Data
A2298-13E
Antiplasmin, alpha2 (HRP)
Description:
Alpha 2Antiplasmin (alpha,2AP), also known as alpha 2Plasmin Inhibitor (alpha 2PI), is a member of the SERPIN family of proteinase inhibitors and the primary inhibitor of the enzyme plasmin in blood. It is produced in the liver and circulates in plasma at ~70ug/ml (~1uM). alpha 2AP is a single chain molecule with a mass of 67kD as determined by SDS-PAGE. The primary target enzyme for alpha 2AP is plasmin, but alpha 2AP also acts as secondary or “backup” inhibitor of activated F.XI, activated Protein C and trypsin. Inhibition of these enzymes by alpha 2AP occurs by proteolytic cleavage after Arg364 with subsequent rapid formation of a stable, inactive 1:1 enzyme-alpha 2AP complex. alpha 2AP also acts to regulate fibrinolysis by binding to the lysine binding sites on plasminogen thus competitively inhibiting plasminogen binding to fibrin. About 30% of alpha 2AP present in plasma is partially degraded and lacks a peptide in the carboxyl region that contains the plasminogen-binding site. This form of alpha 2AP (~65kD) has a reduced rate of plasmin inhibition and has been referred to as the “slow form” of alpha 2AP. During fibrin formation, a portion of circulating alpha 2AP is cross-linked to the alpha -chain of fibrin by activated factor XIII, and this linking of plasmin inhibitor to the plasmin substrate provides an additional measure of protection to the fibrin clot from proteolysis by plasmin.

Applications:
Suitable for use in Immunoelectrophoresis and ELISA. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Rz ratio (Reinheitszahl, A403/A280): 0.485

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ug-20°CBlue IceHumanGoat
Concentration:
~2mg/ml
Immunogen:
Human alpha2 Antiplasmin (alpha2AP) purified from plasma
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in a buffered stabilizer solution, 50% glycerol. No preservatives added. Labeled with HRP.
Specificity:
Recognizes human Antiplasmin, alpha2. Assay by Immunoelectrophoresis has one arc with normal plasma.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Aoki N, Suni Y, Miura O, Hirosawa S; Human alpha 2Plasmin Inhibitor; Methods in Enzymology, 223, pp 185-197, 1993. 2. Shieh BH, Travis J; JBC 262, pp 6055-6059, 1987. 3. Moroi M, Aoki N; JBC 251, pp 5956-5965, 1976. 4. Harpel PC; Blood Proteolytic Enzyme Inhibitors: Their Role in Modulating Blood Coagulation and Fibrinolytic Enzyme Pathways; in Hemostasis and Thrombosis, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 738-747, J.B. Lippincott Co., Philadelphia PA, USA, 1982.