Description:
Applications:
Suitable for use in ELISA (as secondary antibody). Other applications have not been tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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| Type | Isotype | Clone | Grade |
|---|
| Mab | IgG1 | 5F85 | Affinity Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 500ug | | 4°C (-20°C Glycerol) | Blue Ice | Human | Mouse |
|
| Concentration: |
| ~1mg/ml |
| Immunogen: |
| Purified human alpha-2-antiplasmin. |
| Purity: |
| Purified by Protein A affinity chromatography. The purity of the product was tested by Laemmli SDS-PAGE. |
| Form |
| Supplied as a lyophilized powder in PBS, pH 7.4, 20mg/ml mannitol, 0.02% sodium azide. Reconstitute with 500ul sterile 40-50% glycerol, dH2O. |
| Specificity: |
| Binds native a-2-antiplasmin and degraded a-2-antiplasmin as well as plasmin-a-2-antiplasmin complexes. Half maximal binding to coated a-2-antiplasmin at ~9ng/ml. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
1. E. Hattey, J. Wojta and B.R. Binder. Monoclonal antibodies against Plasminogen and ¬-2-plasminogen: Binding to native and modified antigens. Thrombosis Research 45:485-495 (1987)
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