Technical Data
A2298-18
Antithrombin III (ATIII) (HRP)
Description:
Antithrombin, also known as Antithrombin III (ATIII), is a member of the SERPIN family of proteinase inhibitors and the primary inhibitor of thrombin in plasma. It is produced in the liver and circulates in plasma at ~200ug/ml (~3.5uM). Antithrombin inhibits a broad spectrum of serine proteases including thrombin, activated forms of factor X, factor IX, factor XI, factor XII, as well as kallikrein, plasmin and urokinase. Enzyme inhibition by antithrombin occurs through proteolytic cleavage at Arg385-Ser386 and subsequent rapid formation of a stable, inactive 1:1 enzyme-antithrombin complex. Heparin has a profound accelerating effect on the inhibitory activity of antithrombin towards some enzymes. For example, the rate of inhibition of thrombin and activated factor X is increased 1000-fold in the presence of optimal concentrations of heparin, whereas heparin has relatively little effect on the inhibition rate of activated factor XI, activated factor XII and kallikrein. Antithrombin is a single chain molecule with a molecular weight of 59kD. Interaction with thrombin results in an SDS-stable thrombin-antithrombin complex of 96kD.

Applications:
Suitable for use in ELISA and Immunoelectrophoresis. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Rz Ratio (Reinheitszahl, A403/A280):
0.422 as determined spectrophotomerically.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanSheep
Concentration:
~1mg/ml
Immunogen:
Human Antithrombin-III (ATIII) purified from plasma.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in a buffered stabilizer solution, 50% glycerol. Labeled with horseradish peroxidase (HRP).
Specificity:
Recognizes human Antithrombin III. Analyzed by IEP, resulted in one arc with normal human plasma and no arc with ATIII deficient plasma.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Damus, P.S. & Rosenberg, R.D., Methods in Enzymology 45: 653-669 (1976). 2. Harpel, P.C., Blood Proteolytic Enzyme Inhibitors: Their Role in Modulating Blood Coagulation and Fibrinolytic Enzyme Pathways; in Hemostasis and Thrombosis, eds. R.W. Colman, J. Hirsh, V.J. Marder & E.W. Salzman, pp. 738-747, J.B. Lippincott Co., Philadelphia PA, USA (1982). 3. Griffith, M.J., et al., JBC 260: 2218-2225 (1985).