Technical Data
A2298-20F
Antithrombin III (ATIII)
Description:
Antithrombin III (ATIII) is a single chain glycoprotein of molecular mass 58kD with four glycosylation sites and three intra-molecular disulfide bonds. AT III is normally present in human plasma at a concentration of approx. 12.5mg/dl. It is the major inhibitor of thrombin. Inactivation of thrombin by AT-III occurs by formation of a covalent bond resulting in an inactive 1:1 stoichiometric complex between the two components.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 1:32,000
Optimal dilutions to be determined by the researcher.

Hybridoma:
X63-Ag8.653. Female CF1 x Balb/c mice immunized i.p. with immunogen adsorbed onto Al(OH)3.

Culture Medium:
RPMI 1640 with 10% fetal calf serum.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k6F28Affinity Purified
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Antithrombin III isolated from human plasma.
Purity:
Purified by Protein A/G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Specificity:
Recognizes human ATIII, native form. Epitope specificity differs from those of clones 6F27 and 6F218. Strong reaction is seen in ELISA with native ATIII coated directly onto the microtiter well, and also when used as detection antibody in sandwich ELISA in combination with a polyclonal capture antibody. Reacts with ATIII in Western Blot after SDS-PAGE.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: Marchand, C. et al., (2010) J Biomed Mater Res A. 93:1429-1441. 1. Sorensen PJ, Ezban M, Jessen TE, Sas G, Blasko G, Jorgensen M (1990) Purification and characterisation of the pathological antithrombin III "Aalborg". Thromb Res 57:807-812.