Technical Data
Antithrombin III (ATIII)
Antithrombin III (ATIII) is a plasma protein synthesized in the liver. Normal plasma levels are 115-160mg/L. ATIII consists of a single polypeptide chain and has a molecular weight of 58kD. ATIII is a serine protease inhibitor and through this action regulates blood coagulation. ATIII also has a heparin binding site and in the presence of heparin the inhibition of thrombin, factors IXa, Xa and XIa is enhanced 1000-fold.

Suitable for use in Radial Immunodiffusion (RID), Double Diffusion and Immunoelectrophoresis. Other applications have not been tested.

Recommended Dilutions:
RID: 6ul antiserum/cm2 gel vs. 5ul neat - 1:10 dilution human plasma.
Double Diffusion: 10ul antiserum vs. 5ul plasma
IEP: 100ul antiserum vs. 5ul plasma gives single arc.
Note: The use of 3% PEG 6000 with 1.2% agarose in a suitable buffer (such as TBE or Tris-barbital pH > 8.2) is recommended.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGHighly Purified
1ml-20CBlue IceHumanSheep
~10mg/ml (OD280nm, E1%=14.5)
Human Antithrombin III purified from plasma.
Purified by DEAE chromatography.
Supplied as a liquid in glycine buffered saline, pH 7.4, 0.099% sodium azide, 0.1% EACA, 0.01% benzamidine, 1mM EDTA.
Recognizes human Antithrombin III (ATIII). Species Crossreactivity: feline, canine, equine, mouse and rat. Does not react with chicken, bovine, porcine, guinea pig, goat or rabbit. Reactivity against the sera of the listed species has been assessed by double diffusion using 10ul of antisera against 5ul of species sera.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.