Technical Data
A2298-28
a1-Antitrypsin (AAT, alpha-1-AT, alpha1 Proteinase Inhibitor, alpha1 PI) (HRP)
Description:
Alpha-1-antitrypsin (AAT) consists of a single polypeptide chain and has a molecular weight of 51kD. It exists in a number of genetic variants. MM variant is the most common. Alpha-1-AT is synthesized in the liver and it acts as an inhibitor of proteases such as trypsin, elastase, chymotrypsin, collagenase, leukocytic proteases, plasmin, and thrombin, which may be released during inflammatory reactions in the lung. In the absence of AAT, these enzymes are not inhibited and they may digest pulmonary parenchyma. AAT deficiency is associated with chronic obstructive lung disease (emphysema) and less frequently with hepatic cirrhosis in infants and respiratory distress of the newborn. Increase in AAT occurs as an acute phase response to tissue necrosis and inflammation. Serum level of alpha-1-AT is elevated in rheumatoid arthritis, bacterial infections, vasculitis, and carcinomatosis. In colorectal and lung carcinomas, AAT has also related to their invasive and metastatic capacity. The average level of AAT in plasma is 1.3g/L.

Applications:
Suitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:500-1:2500
ELISA: 1:1000-5000
Immunohistochemistry: 1:200-1:1000
Optimal dilution determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
TypeIsotypeCloneGrade
PabIgGHighly Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~1mg/ml (after reconstitution)
Immunogen:
alpha1-Antitrypsin from human plasma.
Purity:
Purified by delipidation, salt fractionation, ion exchange chromatography and dialysis.
Form
Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease Free), 0.01% gentamycin sulfate. Labeled with horseradish peroxidase (HRP). Reconstitute with 100ul sterile ddH2O.
Specificity:
Recognizes human alpha1-Antitrypsin. Assay by IEP resulted in a single precipitin arc against anti-peroxidase and anti-goat serum as well as purified and partially purified alpha1-Antitrypsin (human plasma).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application references: 1. Huang, T.-K. et al., (2008) Biotechnology and Bioengineering 102:508-520. 2. Huang, T.-K. et al., (2010) Biotechnology and Bioengineering 106; 408-421. General Reference(s): 1. Farr & Nakane, J. Immunol. Methods 47: 129-144 (1981).