		Technical Data
A2298-28B	a1-Antitrypsin (AAT, alpha-1-AT, alpha1 Proteinase Inhibitor, alpha1 PI) (HRP)

Description:
Alpha-1-antitrypsin (AAT) consisting of a single polypeptide chain and has a molecular weight of 51kD. It exists in a number of genetic variants. MM variant is the most common. Alpha-1-AT is synthesized in the liver and it acts as an inhibitor of proteases such as trypsin, elastase, chymotrypsin, collagenase, leukocytic proteases, plasmin, and thrombin, which may be released during inflammatory reactions in the lung. In the absence of AAT, these enzymes are not inhibited and they may digest pulmonary parenchyma. AAT deficiency is associated with chronic obstructive lung disease (emphysema) and less frequently with hepatic cirrhosis in infants and respiratory distress of the newborn. Increase in AAT occurs as an acute phase response to tissue necrosis and inflammation. Serum level of alpha-1-AT is elevated in rheumatoid arthritis, bacterial infections, vasculitis, and carcinomatosis. In colorectal and lung carcinomas, AAT has also related to their invasive and metastatic capacity. The average level of AAT in plasma is 1.3g/L.

Applications:
Suitable for use in Immunofluorescence, ELISA, Western Blot, Dot Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:4000-1:14,000
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.

Type	Isotype	Clone	Grade
Pab	IgG		Purified

Size		Storage	Shipping	Source	Host
4mg		-20°C	Blue Ice	Human	Goat

Concentration:

~1mg/ml

Immunogen:

a1-Anti-Trypsin fom human plasma. Cellular Localization: Secreted

Purity:

Purified

Form

Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.01% gentamicin sulfate. Labeled with Horseradish Peroxidase (HRP).

Specificity:

Recognizes human alpha1-antitrypsin. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-goat serum as well as purified and partially purified alpha1-anti-trypsin (human plasma).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

Farr AG & Nakane PK Immunohistochemistry with enzyme labeled antibodies: a brief review. J Immunol Methods 47:129-44 (1981).