		Technical Data
A2298-48B	AP1g1 (AP-1 Complex Subunit gamma-1, Adapter-related Protein Complex 1 Subunit gamma-1, Golgi Adaptor HA1/AP1 Adaptin Subunit gamma-1, Clathrin Assembly Protein Complex 1 gamma-1 Large Chain)

Description:
Adaptins are important components of clathrin-coated vesicles transporting ligand-receptor complexes from the plasma membrane or from the trans Golgi network to lysosomes. Together with medium and small subunits, adaptins form a heterotetrameric complex called an adaptor whose role is to promote the formation of clathrin-coated pits and vesicles and to recognise sorting signals within the cytosolic tails of transmembrane cargo molecules. Gamma-adaptin protein belongs to the adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network and/or endosomes.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:4000
Immunohistochemistry (Frozen, paraffin): 1:200-1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Serum

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

Not determined

Immunogen:

Synthetic peptide corresponding to aa5-24, IRLRELIRTIRTARTQAEER of human AP1g1 conjugated to diphtheria toxin. Cellular Localization: Golgi apparatus. Cytoplasmic vesicle, clathrin-coated vesicle membrane.

Purity:

Serum

Form

Supplied as a lyophilized powder. Reconstitute in 100ul sterile ddH2O.

Specificity:

Recognizes. human AP1g1. Species Crossreactivity: rat. Species Sequence Homology: mouse, canine, zebrafish

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

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