Technical Data
A2298-48B
AP1g1 (AP-1 Complex Subunit gamma-1, Adapter-related Protein Complex 1 Subunit gamma-1, Golgi Adaptor HA1/AP1 Adaptin Subunit gamma-1, Clathrin Assembly Protein Complex 1 gamma-1 Large Chain)
Description:
Adaptins are important components of clathrin-coated vesicles transporting ligand-receptor complexes from the plasma membrane or from the trans Golgi network to lysosomes. Together with medium and small subunits, adaptins form a heterotetrameric complex called an adaptor whose role is to promote the formation of clathrin-coated pits and vesicles and to recognise sorting signals within the cytosolic tails of transmembrane cargo molecules. Gamma-adaptin protein belongs to the adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network and/or endosomes.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:4000
Immunohistochemistry (Frozen, paraffin): 1:200-1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to aa5-24, IRLRELIRTIRTARTQAEER of human AP1g1 conjugated to diphtheria toxin. Cellular Localization: Golgi apparatus. Cytoplasmic vesicle, clathrin-coated vesicle membrane.
Purity:
Serum
Form
Supplied as a lyophilized powder. Reconstitute in 100ul sterile ddH2O.
Specificity:
Recognizes. human AP1g1. Species Crossreactivity: rat. Species Sequence Homology: mouse, canine, zebrafish
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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