Technical Data
AP2m1 (Adaptor-related Protein Complex 2 mu 1)
AP2M1 is a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunit family. AP2 is a heterotetramer composed of two large adaptins (either alpha-type subunit AP2A1 or AP2A2 paired with beta-type subunit AP2B1), a medium adaptin (mu-type subunit AP2M1) and a small adaptin (sigma-type subunit AP2S1). These component link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration.

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 0.05-0.2ug/ml
Western Blot: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute by adding sterile 40-50% glycerol ddH2O. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
40ug-20CBlue IceHumanRabbit
Synthetic peptide from amino terminal of human AP2M1 protein (Swiss Prot: Q96CW1). (KLH).
Purified by immunoaffinity chromatography.
Supplied as a lyophilized powder from PBS, pH 7.4, 0.02% sodium azide.
Recognizes endogenous levels of human AP2M1 protein. Species sequence homology: rat and mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.