Technical Data
A2298-51D
AP2m1 (Adaptor-related Protein Complex 2 mu 1)
Description:
AP2M1 is a subunit of heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complex medium subunit family. AP2 is composed of two large adaptins (alpha-type subunit AP2A1, or AP2A2, and beta-type subunit AP2B1), one medium adaptin (mu-type subunit AP2M1), and a small adaptin (sigma-type subunit AP2S1). The AP2 complex is part of the protein coat linking clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins so that they can be selected and concentrated.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 0.05-0.2ug/ml
Western Blot: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
40ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide . within residues 150-200 of human AP2M1 protein (KLH) (Swiss Prot: Q96CW1).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 0.02% sodium azide.
Specificity:
Recognizes human AP2M1 protein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.