Technical Data
A2298-51T
AP2S1 (Adaptor-related Protein Complex 2 sigma 1 Subunit, AP2 Complex Subunit sigma 1, AP-2 Complex Subunit sigma 1, AP 17, AP17, AP17 delta, Clathrin Assembly Protein 2 Small Chain, Clathrin-associated/assembly/adaptor Protein Small 2, CLAPS2, Clathrin C
Description:
Adapter-related protein complex 2 sigma-1 subunit (AP2S1, AP17) is the component of the adaptor complexes which links clathrin to receptors in coated vesicles.It is the smallest polypeptide chain component of AP-2, a heterotetramer composed of two large adaptins (alpha-type subunit, AP2 alpha, and beta-type subunit, AP2 beta), a medium adaptin (mu-type subunit, AP2 mu1) and a small adaptin (sigma-type subunit, AP2S1).AP-2 is one of two major clathrin-associated protein complexes that interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration (1, 2).

Applications:
Suitable for use in Flow Cytometry, Western Blot and Immunoprecipitation. Not recommended for use in Immunocytochemistry or Immunohistochemistry (paraffin sections). Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:200
Western Blot: 1:1000-1:5000. Expected to detect a band at ~17kD.
Immunoprecipitation: 1:10
Optimal dilutions to be determined by the researcher.

Recommended Positive Control:
Fetal brain lysate, HeLa cell lysate

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG9H292Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not determined.
Immunogen:
A synthetic peptide corresponding to residues in human AP2S1.
Purity:
Supernatant
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.01% sodium azide, 50% glycerol.
Specificity:
Recognizes human AP2S1. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kirchhausen T, et al. J Biol Chem. 266(17):11153-7, 1991 2. The UniProt Consortium, The Universal Protein Resource (UniProt). Nucleic Acids Res. 36:D190-D195 (2008)