Technical Data
AP3s1, Recombinant, Human (Adaptor-related Protein Complex 3 sigma 1, Clathrin Assembly Protein Complex 3)
Molecular Biology Storage: -20CShipping: Blue Ice
Part of the AP-3 complex, an adapter-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes.

Recombinant Human AP3S1 produced in E.Coli is a non-glycosylated, polypeptide chain containing amino acids (1-193) and having a total molecular mass of 22.2kD. Recombinant AP3S1 contains T7 tag at N-terminus and is purified by proprietary chromatographic techniques.

ELISA MS Western Blotting

Recombinant P3S2 although stable at 140C for 1 week, should be stored desiccated below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Molecular Weight:
Source: E. coli
Purity: 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
Concentration: ~0.1mg/ml
Form: Supplied as a liquid in 10mM Tris, pH 8.0, 0.01% Triton X-100, 0.002% sodium azide and 10mM DTT.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Localization of the AP-3 adaptor complex defines a novel endosomal exit site for lysosomal membrane proteins. J Cell Biol 2004 Mar 29;164(7):1065-76 2. Role of adaptor complex AP-3 in targeting wild-type and mutated CD63 to lysosomes. Mol Biol Cell 2002 Mar;13(3):1071-82 3. The assembly of AP-3 adaptor complex-containing clathrin-coated vesicles on synthetic liposomes. Mol Biol Cell 2000 Nov;11(11):3723-36 4. Distinct requirements for the AP-3 adaptor complex in pigment granule and synaptic vesicle biogenesis in Drosophila melanogaster. Mol Gen Genet 2000 Jul;263(6):1003-14 5. Trafficking of major histocompatibility complex class II molecules in human B-lymphoblasts deficient in the AP-3 adaptor complex. Immunol Lett 2000 May 1;72(2):113-7 6. Defective expression of the mu3 subunit of the AP-3 adaptor complex in the Drosophila pigmentation mutant carmine. Mol Gen Genet 1999 Oct;262(3):401-12a

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.