Technical Data
A2298-65S
APAF1, CT (Apoptotic Protease-activating Factor 1, APAF-1, KIAA0413)
Description:
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologous of the key cell death gene CED-4 in C. elegans was identified recently from human and mouse and designated Apaf1 for apoptosis protease- activating factor 1. Apaf1 binds to cytochrome C (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 transcript is ubiquitously expressed in human tissues.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human heart tissue lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
~0.1mg/ml
Immunogen:
Synthetic peptide corresponding to aa near the C-terminus of human Apaf1.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH7.4, 0.05% sodium azide.
Specificity:
Recognizes human Apaf1. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Zou, H. et al. Cell 1997; 90:405-13. 2. Cecconi, F. et al. Cell 1998; 94:727-37. 3. Li, P. et al. Cell 1997; 91:479-89. 4. Hu, Y. et al. Proc Natl Acad Sci USA 1998; 95:4386-91.