Technical Data
APE (Ref-1, Apurinic Apyrimidinic Endonuclease, APEX, APE-1, HAP1)
The mammalian apurinic/apyrimidinic endonuclease
(APE/ref-1) is responsible for the repair of AP sites in DNA. In addition, this enzyme functions as a redox factor facilitating the DNA binding capability of FOS, JUN, NfkB, HIF-1 alpha, Pax-5, Pax-8 and other transcription factors. APE/ref-1 has also been shown to control p53 activity through redox alteration. APE is linked to apoptosis, associated with thioredoxin, and altered levels of APE/ref-1 have been found in some cancers. APE appears to form a unique link between the DNA base excision pathway, oxidative signaling, transcription regulation, cancer and cell-cycle control.

Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.

Recommended Dilution:
Western Blot: > 1:2000 Detects single 37kD band using ECL. Immunohistochemistry (frozen and paraffin fixed sections and cytospin preps): > 1:5000, it can be competitively inhibited from recognizing APE/ref-1 antigen in tissues using APE/ref-1 protein.
Optimal dilution determined by the researcher.

Positive Control:
HeLa whole cell extract

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
50ul4C (-20C Glycerol)Blue IceHumanMouse
Not determined
Purified human APE/ref protein. Epitope: amino acids 80-100. Cellular Localization: In Immunohistochemistry, punctate nuclear staining is observed in most cell types. Cytoplasmic staining is seen in hepatocytes, large neurons and granule cells.
Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.
Recognizes human APE/ref-1 protein. Species Crossreactivity: Human, mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Evans, A.R., et al., Mutation Res. 461: 83-108 (2000). 2. Hedley, D., et al., Am. J. Pathol. 164: 557-565 (2004).